Analysis offer of CIRAD: Checking the conformity of Hevea budwood gardens by microsatellite DNA analysis (Rubber ID)

For rubber growers, checking clonal identity in budwood gardens is essential. Prior to planting out, it ensures the trueness-to-type of the improved planting material chosen by the grower for its yield potential, agronomic qualities, or disease resistance. A clonal error will have effects over the 25 to 30 years’ exploitation of the plots involved. Checking the quality of propagated planting material therefore has a direct impact on the economic performance of plantations and the ecological intensification of yields per area of land.

The DNA analysis method which was initially called Misathev, based on 8 microsatellite markers, can be used with certainty to establish a profile for each plant, comprising 8 pairs of alleles. Over the last 15 years, CIRAD researchers have used it to index over 1,500 genotypes, each with a unique profile, thereby enabling identification by comparison of the great majority of Hevea clones existing in plantations .

Seeking to share these advances with industry, this approach has been part of CIRAD’s appraisal services since 2010, based on analyses of individual samples enabling growers to check, with total certainty, the identity of certain stumps. As it was judged useful and efficient, Misathev benefited from further developments to broaden its use.

In order to take into account the diversity of situations in budwood gardens, CIRAD proposes two options in -Rubber ID-: analyses by batches of 5 or 10 individuals.

Depending on the situations, the main results provided by batch analysis may be as follows:

  • Batch found to be “homogeneous” => establishment of a single profile, and certification of the corresponding clone for all the samples in the batch
  • Batch found to be “heterogeneous-single”, with only one contaminant => identification of the predominant clone, of the contaminant clone, and of the relative importance of its presence
  • Batch found to be “heterogeneous-complex” => indication of the existence of a complex situation generated by major and multiple contamination
  • The traceability ensured by the laboratory provides the possibility of returning to individual analysis to establish the precise composition of heterogeneous batches.

On this basis, the analysis options offered to growers have been defined and are presented here for the submission of individual or batch samples, depending on the situation and objectives. 

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